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KMID : 0613820130230080970
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Journal of Life Science
2013 Volume.23 No. 8 p.970 ~ p.977
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Early Growth Response 1 Induces Epithelial-to-mesenchymal Transition via Snail
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Jeon Hyun-Min
Lee Su-Yeon
Ju Min-Kyung
Park Hye-Gyeong
Kang Ho-Sung
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Abstract
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The epithelial-to-mesenchymal transition (EMT) plays an essential role in embryogenesis and is involved in tumor metastasis and invasion; it significantly contributes to tumor progression and aggressiveness. The EMT is characterized by a loss of epithelial cell polarity as a result of the reduced expression of epithelial E-cadherin, a hallmark of the EMT, and the acquisition of mesenchymal-like cell morphology. Reactive oxygen species (ROS) such as O₂^-, H₂O₂and OH^- have been demonstrated to induce the EMT; although Snail is involved in ROS-induced EMT by transcriptionally repressing E-cadherin, its mechanism is not fully understood. In this study, we examined the effects of early growth response 1 (Egr-1) overexpression in noninvasive breast tumor cell line MCF-7 cells. Upon Egr-1 overexpression, MCF-7 cells lost epithelial cell polarity and became more spindle-shaped, indicating that Egr-1 may induce EMT. We found that Snail is implicated in Egr-1 induced EMT. We further demonstrate that the Egr-1-Snail axis is activated by ROS and plays a critical role(s) in ROS-induced EMT.
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KEYWORD
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Egr-1, epithelial-to-mesenchymal transition (EMT), Snail, E-cadherin, reactive oxygen species (ROS)
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